One woman was taken to hospital and two other adults were treated by medics after fainting in Stevenage, Herts.
And in a separate case, a man collapsed at a cinema in Peterborough, Cambs, "due to the film's content".
"If you know you're squeamish, don't go," warned a spokesman for the East of England Ambulance Service.
"This is obviously a blood-thirsty film. Some of the scenes are fairly horrific," Matthew Ware told the BBC News website.